Synergy™ H1m is a flexible monochromator-based multi-mode microplate reader. The monochromator optics use a third generation quadruple grating design that works at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories. It has the following features:
- A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).
- User-adjustable orbital shaking and advanced 4-Zone™ temperature control
- Compatible with Take3™ Micro-Volume Plates: Samples down to 2 μL volume can be measured. Especially useful when working with precious samples, for fast and accurate DNA/RNA quantification at 260 nm
Video Introduction from
| GENERAL | |
| Detection modes | UV-Vis absorbance |
| Fluorescence intensity | |
| Luminescence | |
| Fluorescence polarization | |
| Time-resolved fluorescence | |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported | Petri and cell culture dishes |
| Take3 Micro-Volume Plates | |
| Temperature control | 4-Zone™ incubation to 45 °C with Condensation Control™ |
| +0.2 °C at 37 °C | |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5™ Data Analysis Software |
| Gen5 Secure for 21 CFR Part 11 compliance (option) | |
| Automation | BioStack and 3rd party automation compatible |
| BioSpa 8 Automated Incubator compatible | |
| CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2) |
| Control Resolution: +0.1% (CO2 and O2) | |
| Stability: +0.2% at 5% CO2; +0.2% at 1% O2 | |
| Models for both CO2 and O2 or CO2 only are available | |
| ABSORBANCE | |
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 - 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 - 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | ±2 nm |
| Monochromator wavelength repeatability | ±0.2 nm |
| OD accuracy | <1% at 2.0 OD |
| <3% at 3.0 OD | |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 11 seconds |
| 384 wells: 22 seconds | |
| FLUORESCENCE INTENSITY | |
| Light source | Xenon flash |
| Detector | PMT for monochromator system |
| PMT for filter system | |
| Wavelength selection | Quad monochromators (top/bottom) |
| Filters (top) | |
| Wavelength range | Monochromators: 250 - 700 nm (850 nm option) |
| Filters: 200 - 700 nm (850 nm option) | |
| Monochromator bandwidth | Fixed, 16 nm |
| Dynamic range | 7 decades |
| Sensitivity | Filters: |
| Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) | |
| Quad Monochromator: | |
| Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top | |
| Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom | |
| Reading speed (kinetic) | 96 wells: 11 seconds |
| 384 wells: 22 seconds | |
| LUMINESCENCE | |
| Wavelength range | 300 - 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | Monos: 20 amol ATP (flash) |
| Filters: 10 amol ATP (flash), 100 amol (glow) | |
| FLUORESCENCE POLARIZATION | |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 280 - 700 nm (850 nm option) |
| Sensitivity | 1.2 mP standard deviation at 1 nm fluorescein |
| TIME-RESOLVED FLUORESCENCE | |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (secondary mode) |
| Filters (top) | |
| Wavelength range | Filters: 200 - 700 nm (850 nm option) |
| Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) |
| Monos: Europium 1200 fM (120 amol/well, 384-well plate) | |
| REAGENT DISPENSERS | |
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well microplates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 - 1000 µL in 1 µL increment |
| Dispense accuracy | ±1 µL or 2% |
| Dispense precision | <2% at 50-200 µL |
| PHYSICAL CHARACTERISTICS | |
| Power | 130 Watts max. |
| Dimensions | 15.4"W 18.6"D 12.9"H |
| (39.1 x 47.2 x 32.8 cm) | |
| Weight | 50 lbs (22.5 kg) |