The Coy Anaerobic Chamber is designed especially for anaerobic microbiology study. The chamber operates with a hydrogen gas mix reacting with a palladium catalyst to remove excess oxygen. The gas mix with H2 gas is circulated through the catalyst and removes O2 by forming a water molecule. Generally, O2 levels equilibrate to 0-5 parts per million (ppm). After initial establishment of the anaerobic atmosphere, the gas mix (H2 5%) should be refreshed every 5-10 days and the catalyst rejuvenated by heating it. The airlock is used to reduce O2 levels prior to the transfer of samples in and out of the chamber to avoid large spikes of O2 into the system.