Curriculum Vitae: Jingjie Hao

CURRICULUM VITAE


CURRICULUM VITAE

Jingjie Hao
Ph.D., M.S.
 

 

 

EDUCATION

  • Iowa State University, Ames, IA 

    • 2009-2014 
      Ph.D. of Genetics, Department of Horticulture 

    • 2011-2012
      Minor of Business Administration, College of Business 

  • China Agricultural University, Beijing, China 

    • 2007-2009
      Master of Vegetable Science, Department of Agriculture 

    • 2003-2007
      Bachelor of Horticulture, Department of Horticulture

 

 

PROFESSIONAL POSITIONS

  • University of Nebraska-Lincoln, Lincoln, NE

    • 2023-Present
      Director of the Biomedical and Obesity Research Core 

    • 2022-Present
      Research Assistant Professor 

    • 2018-2022
      Post-Doctoral Research Associate 

  • Viosimos Agriculture LLC, Lincoln, NE

    • 2022-Present
      Research Scientist (0.5 FTE) 

  • University of California, Davis, CA 

    • 2015-2017
      Laboratory Manager (Research Associate) (0.5 FTE) 

  • Forage Genetics International, Davis, CA 

    • 2015-2017
      Research Associate (0.5 FTE) 

    • 2017
      Acting Laboratory Manager

 

 

RESEARCH EXPERIENCE & ACTIVITIES

  • University of Nebraska-Lincoln, Lincoln, NE

    • Director of the Biomedical and Obesity Research Core
      2023-Present

      • Oversee the core’s day-to-day operations, serving as the primary contact for biostatistics coordinators and the
        Holland Computing Center to ensure seamless core support

      • Conduct user satisfaction surveys; Coordinate equipment upgrades along with maintenance and repairs

      • Ensure the core’s compliance with federal, state, and university policies and regulations

      • General management of the core budget

    • Research Assistant Professor
      2022-Present

      • Coordinated and led a collaborative soil microbiome project for a maize diversity panel. Managed all aspects of the project, including experimental planning, personnel and timeline coordination, sample collection, processing, and data analysis 

      • Generated high-quality MiSeq data of microbial communities by creating a large number of 16S rRNA sequencing libraries with samples from the aforementioned project

      • Characterized the functions of CCD genes in strigolactones biosynthesis and plant-microbe interactions through CRISPR/Cas9 in sorghum 

    • Undergraduate Creative Activities and Research Experiences (UCARE) selection committee
      2022-Present

      • Assess and evaluate research project proposals submitted by undergraduate students 

  • Viosimos Agriculture LLC, Lincoln, NE

    • Research Scientist
      2022-Present

      • Identify potential target pathogens, modes of action, and strategies for fungal disease control in leafy green

      • Design and implement research protocols to identify approaches for the development of biofungicide

      • Establish connections and collaborations with external research institutions and consultants. Monitor and stay informed about industry trends and advancements in the field of biofungicides

      • Ensure compliance with relevant regulatory guidelines and accommodate regulations related to the develop ment, testing, and commercialization of biofungicides

  • University of Nebraska-Lincoln, Lincoln, NE

    • Post-Doctoral Research Associate
      2018-2022

      • Optimized 16S rRNA sequencing library preparation protocol to generate high-quality MiSeq data of the microbial communities for soil, rhizosphere and plants

      • Conducted microbiome analysis investigating the functions and changes in bacterial and fungal communities in diverse environments

      • Investigated the effects of microbial interactions on maize root functions through transcriptomic analysis

      • Performed data analysis on high-throughput phenotyping data generated from an automated plant phenotyping platform in soybean to quantify plant traits non-destructively

  • University of California, Davis, CA

    • Research Associate
      2015-2017

      • Generated high-quality genotyping data for studying plant fall dormancy using optimized Genotyping-by Sequencing (GBS) protocol on alfalfa

      • Optimized spiked GBS (sGBS) protocol for alfalfa, generated high-quality data for QTL mapping studies 

  • Forage Genetics International, Davis, CA

    • Research Associate
      2015-2017

      • Performed large-scale production genotyping assays to identify alfalfa plants that conform standard quality criteria for forage production

      • Maintained the routine genotyping and screening procedure for alfalfa to ensure smooth operations 

  • Iowa State University, Ames, IA

    • Graduate Research Assistant
      2009-2014

      • Established stable Agro-mediated plant transformation systems for Brachypodium and bentgrass 

      • Conducted differential expression analysis of cold-responsive genes in BdCBF3 mutants using RNA-seq

      • Constructed a salt stress-specific RNAi library from a cDNA library in creeping bentgrass

    • Service Learning Chair of MBA Association
      2011-2012

      • Coordinated on community service projects and social activities for the MBA Association

  • China Agricultural University, Beijing, China

    • General Affairs Chair of Graduate Student Association
      2007-2009

      • Organized cultural and recreational activities, and contributed to class discipline construction procedures

    • Academic Exchange to Japan Hirosaki University
      August 2007

      • Engaged in scientific and cultural exchange with university students and professors

 

 

PROFESSIONAL SKILLS

Sequencing Library Construction and Genotyping 

  • Construction of Genotyping-by-Sequencing (GBS) library and Restriction site Associated DNA (RAD) library for Illumina HiSeq 

  • TaqMan-based large-scale SNP genotyping and copy number assays 

  • Preparation of 16S rRNA library for Illumina MiSeq 

  • Construction of RNAi and cDNA libraries 

Wet Laboratory Experience 

  • DNAandRNAisolation, reverse transcription, PCR, quantitative real-time PCR, Southern blot 

  • Construction of CRISPR and RNAi vector, including primer design, digestion, ligation, and cloning 

  • Bacterial and fungal culture, plasmid isolation and transformation 

  • RNAimutagenesis and gene expression profiling 

  • Plant transformation and tissue culture for various plant species (eg., Brachypodium and bentgrass) 

  • Operation and programming of liquid handling robotics platforms (eg., Beckman, Agilent) 

  • Plant hydroponic culturing and collection of root exudates 

  • Plant maintenance in greenhouse and growth chamber 

  • Operation of automated phenotyping system for plant phenotype data collection 

Computational Skills 

  • Sequencing data management and statistical analysis 

  • Basic bioinformatics and data processing 

  • Image processing, analysis, and time-series modeling of plant phenotype imaging data 

  • Analysis of 16S rRNA/ITS sequencing data using the QIIME pipeline and R programming language 

Managerial Skills 

  • Efficient project planning, organizing, and execution with clear goals, timelines, and deliverables 

  • Laboratory resource allocation including equipment, supplies, budgets, and personnel 

  • Excellent verbal and written communication skills for effective collaboration and conveying information 

  • Strong supervision and mentoring skills in providing guidance, support, and mentorship to lab members 

  • Knowledge of regulatory requirements and ethical guidelines in biological research activities

 

 

PUBLICATIONS

  1. Hao J, Yang Y, Futrell S, Kelly EA, Lorts CM, Nebie B, Runo S, Yang J, Alvarez S, Lasky JR, Schachtman DP. CRISPR/Cas9-mediated mutagenesis of carotenoid cleavage dioxygenase (CCD) genes in sorghum alters strigolactone biosynthesis and plant biotic interactions. Phytobiomes Journal. (2023) 

  2. Lopes LD, Futrell SL, Bergmeyer E, Hao, J, Schachtman DP. Root exudate concentrations of indole-3-acetic acid (IAA) and abscisic acid (ABA) affect maize rhizobacterial communities at specific developmental stages. FEMS Microbiology Ecology. (2023) 

  3. Hao J, MaC, Yin Y, Fei SZ. A novel method of generating RNAi library for high throughput gene function analysis in creeping bentgrass. International Turfgrass Society Research Journal. (2022) 

  4. Hao J, Chai YN, Lopes LD, Ordóñez R, Wright E, Archontoulis S, Schachtman DP. The effects of soil depth on the structure of microbial communities in agricultural soils in Iowa, USA. Applied and Environmental Microbiology. (2021) 

  5. Lopes LD, Hao J, Schachtman DP. Alkaline soil pH affects bulk soil, rhizosphere and root endosphere microbiomes of plants growing in a Sandhills ecosystem. FEMS Microbiology Ecology. (2021) 

  6. Munjal G, Hao J, Teuber LR, Brummer EC.Selection mapping identifies loci underpinning autumn dormancy in alfalfa (Medicago sativa). G3: Genes, Genomes, Genetics. (2018) 

  7. Hao J, Yang J, Dong J, Fei SZ. Characterization of the BdCBF gene family and genome-wide analyses on BdCBF3-dependent and-independent cold-responsive genes in Brachypodium distachyon. Plant Science. (2017) 

  8. LiY,HanL,HaoJ,FeiSZ.Agrobacteriumtumefaciens-mediatedtransformationofbigbluestem(Andropogon gerardii Vitman). Plant Cell, Tissue and Organ Culture. (2015) 

  9. Hao J. Genomic studies of abiotic stresses in grasses. Ph.D. dissertation. (2014) 

  10. Hao J, Yin Y, Fei SZ. Brassinosteroid signaling network: implications on yield and stress tolerance. Plant Cell Reports. (2013)

 

 

MANUSCRIPTS IN PREPARATION

  1. Hao J, Shi Y, Zhang C, Yu B, Schachtman DP. Elucidating the molecular influence of soil microbes on the maize root transcriptome. 

  2. Hao J, Xu G, Ulutas M, Jin H, Schachtman DP, Yang J. Root-associated microbiomes contribute to phenotypic heterosis in a maize diversity population under varying nitrogen conditions. 

  3. Hao J, Yin Y, Fei SZ. Knockdown of the AsBri1 gene enhances drought tolerance in creeping bentgrass (Agrostis stolonifera L.)

 

 

POSTERS & PRESENTATIONS

  1. Hao J, Yu B, Zhang C, Schachtman DP. Elucidating the molecular influence of soil microbes on the maize root transcriptome. Poster: The American Society of Plant Biologists (ASPB) Conference, August 3-7, 2019 

  2. Munjal G, Hao J, Teuber LR, Brummer EC. Using selection mapping to assess the genetic control of fall dormancy in cultivated Alfalfa (Medicago sativa). Poster: The Plant & Animal Genome XXIV Conference, January 9-13, 2016 

  3. Hao J, Yang J, Dong J, Fei SZ. Transcriptome analysis of a cbf3 mutant by RNA-seq in Brachypodium distachyon. Poster: The Plant & Animal Genome XXIII Conference, January 10-14, 2015 

  4. Hao J, MaC, Fei SZ. Construction of a high throughput salt-specific RNAi library for creeping bentgrass (Agrostis stolonifera L.). Poster: The Plant & Animal Genome XXII Conference, January 11-15, 2014 

  5. Hao J, Dong J, Fei SZ. Knockdown of CBF1 and CBF3 genes reduces the capacity for cold acclimation and freezing tolerance in Brachypodium distachyon. Poster: The Plant & Animal Genome XXII Conference, January 11-15, 2014